The 61-year-old pop superstar - who had to axe a run of shows in Boston, Massachusetts from 30 November to 2 December for health reasons - has turned to osteopath Jean Michel Ete for his radical autohemotherapy rehab fix, which mixes blood with ozone gas.
Alongside a video of her undergoing the process, she wrote: "Infusing the blood… Vitamin drip… Tibetan bowls… singing and meditation… Felt amazing afterwards!! (sic)"
In the clip, the 'Like A Virgin' singer appeared with seven-year-old twin daughters Stella and Estere, who stood by their mum as blood was extracted, mixed with gas and then pumped back into her body using an intravenous drip.
The treatment comes after Madonna had to cancel the three 'Madame X' tour dates as she asked fans for forgiveness.
She said at the time: "Please forgive this unexpected turn of events. Doing my show every night brings me so much joy and to cancel is a kind of punishment for me, but the pain I'm in right now is overwhelming and I must rest and follow doctor's orders so I can come back stronger and better and continue the Madame X journey with all of you."
Promoter Live Nation confirmed the shows will not be rescheduled "due to tight scheduling through the balance of the year" and all tickets will be refunded.
Just a day before the cancellation, the 'Hung Up' hitmaker admitted she had "multiple injuries".
She shared a video on Instagram of her in the bath and wrote: "Celebrated My Last show in L.A with my usual Ice Bath for multiple injuries. [Love] and THANKS to my gang-gang for being my hype squad (sic)"